Journal: BMC Immunology

Article Title: Changes of B cell subsets in central pathological process of autoimmune encephalomyelitis in mice

doi: 10.1186/s12865-019-0301-4

Figure Lengend Snippet: Changes in B cell subsets in EAE mice over 30 day experimental period. The activated B-cells, memory B-cells and plasma cells were determined using markers CD19 + and IgD + (activated B-cell), CD19 + , CD27 + and B220 + (memory B-cell) and CD19 − and CD138 + (plasma cell).* denotes P < 0.05 vs control

Article Snippet: The primary antibodies were antibodies against rabbit IL-4 (bs-0581R, Bioss), IL-17 (bs -1183R), IL2RA/CD25 (bs-0577R), IFN-gamma (bs-0480R), syndecan-1 (bs-1309R), CD27 (bs-2491R), CD19 (bs-4755R), CD4 (bs-0647R) and CD45 (bs-10599R), polyclonal antibody against CD3E (A1753, ABdonal) and plasma cell antibody[LIV3G11] (ab44876, abcam).

Techniques:

Journal: BMC Immunology

Article Title: Changes of B cell subsets in central pathological process of autoimmune encephalomyelitis in mice

doi: 10.1186/s12865-019-0301-4

Figure Lengend Snippet: Immunohistochemical assays ( a ) and relative expression ( b ) of CD19 + cells in the spinal cord and brain tissue of EAE mice (× 200). A and G. control at day 7; B and H, EAE mice at day 7; C and I, control at day 18; D and J, EAE mice at day 18, E and K. control at day 30 and F and L. EAE mice at day 30, respectively. A-F, spinal cord tissue; G-L, brain tissue. Arrows denote CD19 + cells. * denotes P < 0.05 vs control

Article Snippet: The primary antibodies were antibodies against rabbit IL-4 (bs-0581R, Bioss), IL-17 (bs -1183R), IL2RA/CD25 (bs-0577R), IFN-gamma (bs-0480R), syndecan-1 (bs-1309R), CD27 (bs-2491R), CD19 (bs-4755R), CD4 (bs-0647R) and CD45 (bs-10599R), polyclonal antibody against CD3E (A1753, ABdonal) and plasma cell antibody[LIV3G11] (ab44876, abcam).

Techniques: Immunohistochemical staining, Expressing, Mouse Assay

Journal: Biology Open

Article Title: Monitoring and evaluation of the immune status of female Kunming mice maintained in different biosafety level laboratories

doi: 10.1242/bio.035006

Figure Lengend Snippet: Constitution of the lymphocyte subtypes of mouse blood and spleen samples. Mice from the ABSL-2/3/4 labs were euthanized at days 7, 14 and 28 of housing, and blood and spleen samples of each mouse were harvested ( n =4, each time point and each group). Percentages of CD3+, CD4+, CD8+ and CD19+ lymphocytes from blood (A,B) and spleen (C,D) samples were determined by flow cytometry. (A,C) Sorting illustrations of blood sample and spleen sample. (B,D) Statistics of total percentages of CD3+, CD4+, CD8+ and CD19+ lymphocytes of blood samples and spleen samples in each mouse group. The error bars represent the standard deviation of each mouse group ( n =12). The P value was conducted based on Student's t -test.

Article Snippet: Cell surface staining was done using Mouse BD Fc Block and anti-mouse CD3-PerCP (Cat. No. 551163, Lot 7038886, clone 145-2C11), CD4-PE (Cat. No. 553652, Lot 7038886, clone H129.19), CD8-FITC (Cat. No. 553030, Lot 7170636, clone 53-6.7), and CD19-APC (Cat. No. 550992, Lot 7081501, clone 1D3) (BD Biosciences).

Techniques: Flow Cytometry, Standard Deviation

Journal: Frontiers in Immunology

Article Title: CCL22-Producing Resident Macrophages Enhance T Cell Response in Sjögren's Syndrome

doi: 10.3389/fimmu.2018.02594

Figure Lengend Snippet: Comprehensive analysis of chemokine genes of sMΦs. (A,B) Chemokine mRNA expressions of isolated CD11b high and CD11b low sMΦs of the SS model mice at 12 weeks of age were analyzed using PCR-array. Up-and downregulated genes of CD11b high sMΦs compared with CD11b low sMΦs of the SS model mice. The lines indicate 5-fold change in the gene expression. Red dots are upregulated genes, and blue dots are downregulated genes. Data are representative of three independent experiments. (C) CCL22 mRNA expression levels of various tissues of the SS model and control mice at 12 weeks of age were determined using qRT-PCR. Data are presented as mean ± SEM. * p < 0.05 by Student's t -test. n = 5. (D) CCL22 expression of sMΦs in salivary glands of control and SS model mice (left). CCL22 positive cell number (right). Data are representative of five mice in each group and are presented as mean ± SEM. *** p < 0.0005 by Student's t -test. n = 5. Gray shadow is isotype control. (E) Comparison of CCL22 expression between CD11b high and CD11b low sMΦs of the SS model mice at 12 weeks of age. Data are representative of five mice. Gray shadow is isotype control. (F) CCL22-producing sMΦs of the SS model mice were detected by confocal microscopic analysis. Data are representative of five mice. (G) Confocal microscopic analysis of CCL22 expression of EpCAM + epithelial cells, CD3 + T cells, CD19 + B cells, and CD11c + DCs in the salivary gland tissues form SS model mice. Data are representative of three mice. Nuclei were stained with DAPI. (H) Immunohistochenical analysis of CCL22 expression using the salivary gland tissues from SS model mice. The result is representative of three mice. Nuclei were stained with hematoxylin.

Article Snippet: Immune cells were stained using antibodies against FITC-conjugated anti-mouse CD206 (BioLegend, C068C2) and CD11c (eBioscience, N418) mAbs, PE-conjugated anti-mouse MHC class II (Miltenyi Biotec, REA478), CD86 (BD Bioscience, GL1), CD204 (eBioscience, M204PA), CCR2, CX3CR1, CCR4 (BioLegend, SA203G11, SA011F11, and 2G12), PE-Cy5.5-conjugated anti-mouse CD3 and CD19 (TONBO Biosciences, 145-2C11, and 6D5) and 7-Aminoactinomycin D (7-AAD) staining solution (TOMBO Biosciences), PE-Cy7-conjugated anti-mouse CD11b (TONBO Biosciences, M1/70), APC-conjugated anti-mouse F4/80 and CD36 (BioLegend, BM8 and HM36), and APC-Cy7-conjugated anti-mouse CD45.2 (TOMBO, 104) mAbs.

Techniques: Isolation, Gene Expression, Expressing, Control, Quantitative RT-PCR, Comparison, Staining

Journal: Polish journal of veterinary sciences

Article Title: B and T lymphocytes in rabbits change according to the sex and throughout the year.

doi: 10.24425/pjvs.2020.132746

Figure Lengend Snippet: Fig. 1. Lymphocytes B with CD19+ receptor in rabbit males, females and total throughout the year. Legend: a – statistically significant difference between male and female, b – statistically significant difference between seasons (total),

Article Snippet: In rabbit blood, the percentage of B-cells with CD19+ receptor (Serotec, mouse anti rabbit IgM B cell marker, catalogue no. MCA812GA), T-cells with CD5+ receptor (Serotec, mouse anti rabbit CD5, catalogue no. MCA800), their subpopulations – T-cells with receptors CD4+ (Serotec, mouse anti rabbit CD4, catalogue no. MCA799G), CD8+ (Serotec, mouse anti rabbit CD8, catalogue no. MCA1576G) and CD25+ (Serotec, mouse anti rabbit CD8, catalogue no. MCA1119GA) was determined according to the method described by Deptuła et al. (1998).

Techniques:

Journal: Cell Reports Medicine

Article Title: Pyroptotic T cell-derived active IL-16 has a driving function in ovarian endometriosis development

doi: 10.1016/j.xcrm.2024.101476

Figure Lengend Snippet: Increased infiltration of CD4 + T cells into the EE (A) Representative analysis of CD3 and CD56 expression in CD45 + cells isolated from RNE or EE. (B and C) Statistical analysis of the percentages of T cells (CD3 + CD56 − ) (B) and NK cells (CD3 − CD56 + ) (C) within the CD45 + cell population of the RNE (n = 18) or EE (n = 12), unpaired t test. Data represent a pool from multiple independent experiments. (D and E) Representative micrographs of CD3 (D) and CD56 (E) IHC staining in RNE and EE cells; scale bar, 50 μm. Data are from at least 3 independent experiments. (F) Representative analysis of CD4 and CD8 proportions in CD45 + CD3 + CD56 − cells isolated from the RNE or EE. (G and H) Statistical analysis of the percentages of CD4T cells (CD4 + CD8 − ) (G) and CD8T cells (CD8 + CD4 - ) (H) within the CD45 + CD3 + CD56 − cell population, RNE (n = 14) and EE (n = 11), unpaired t test. Data represent a pool from multiple independent experiments. (I‒L) Representative micrographs of CD4 (I), CD8 (J), CD19 (K), and CD14 (L) IHC staining in RNE and EE cells; scale bar, 50 μm. Data are from at least 3 independent experiments. All of the samples used for analysis in this figure were in the secretory phase.

Article Snippet: Anti-Human CD19 (clone D4V4B) , Cell Signaling Technology , Cat# 90176; RRID: AB_2800152.

Techniques: Expressing, Isolation, Immunohistochemistry

Journal: Cell Reports Medicine

Article Title: Pyroptotic T cell-derived active IL-16 has a driving function in ovarian endometriosis development

doi: 10.1016/j.xcrm.2024.101476

Figure Lengend Snippet:

Article Snippet: Anti-Human CD19 (clone D4V4B) , Cell Signaling Technology , Cat# 90176; RRID: AB_2800152.

Techniques: Recombinant, Protein Extraction, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Multiple Displacement Amplification, Staining, Enzyme-linked Immunosorbent Assay, Sequencing, Software